Monoclonal Antibodies .net

Thanks for visiting our website. Our website reviews the production of monoclonal antibodies and the services that we can provide to generate custom antibodies monoclonal. Our company VRISKO LTD is specialized in antibody production  including mouse monoclonal antibody production using hybridoma technology.

Monoclonal antibodies

Production of monoclonal antibodies

There are two different methods for the production monoclonal antibodies: hybridoma technology and recombinant antibody technology. For production of recombinant antibodies visit our website www.protein-engineering.eu or our company website www.vrisko.net.

Production of monoclonal antibodies by the hybridoma technology.

The hybridoma technology was developed by the scientists Niels K. Jerne, Georges Köhler and Cesar Milstein who were awarded Nobel prize in Physiology or Medicine in 1984 for this contribution to the science. The hybridoma technique consists in extracting B lymphocytes from the spleen of a laboratory animal (e.g. mice) that had been first exposed to an antigen to which we are interested in isolating an antibody against. The B cells can secrete antibodies monoclonal but not in a significant quantity and do not survive for long. The specific antibody producing B-cells are fused with myeloma cells which is a type of cancer cell line, which grow indefinitely in cell culture. Also the key in the hybridoma technology is the fusion of the two cell types, one is programmed to produce specific antibodies monoclonal but without the capacity of multiplying indefinitely and the other immortal with high capacity to grow but without the capacity of producing antibodies. The resulting cells, called hybridoma, will have the capacity to multiply quickly and indefinitely and to produce high quantity of monoclonal antibodies. During the process the cells are diluted to obtain one cell per well. The antibodies produced by different clones are analyzed to determine their capacity to bind the antigen. By this way the positive clones are selected and isolated. See a picture of the hybridoma technology. The monoclonal antibodies can be produced in cell culture or in laboratory animals. When the hybridoma cells are injected in the peritoneal cavity of a mouse, it will produce a tumor which synthesize a fluid rich in antibodies called ascitic liquid.

Our antibody production services

VRISKO LTD delivers antibody services of high quality and to a low cost. We are committed to provide the highest level of products and antibody services.

Production of monoclonal antibodies

Production of monoclonal antibodies can be obtained by purchasing our services. We provide high quality production of monoclonal antibodies at a low price.

Mouse Monoclonal Antibody Production

The Custom production of monoclonal antibodies Basic package includes production of monoclonal antibodies by the hybridoma technology. In this package is not included recombinant protein expression, recombinant protein production or peptide synthesis used to humanized the mouse.

Basic package

1. Immunization (5 mice/antigen) and Sera Testing by ELISA.
2. Fusion and Colony Screening.
3. Subcloning, Colony Expansion and Freezing.
4. Guaranteed western blot positive clones (2 clones)
5. Antibody Purification from Ascites by Protein A/G (2 clones, 3mg each)

* Customer to provide plasmid in expression vector with HIS or GST tag. The customer can provide a peptide antigen or a ready-to-inject antigen for monoclonal antibody production. When using a peptide antigen supplied by the customer, an additional charge  is applied for KLH conjugation.

Price: $4200

Cat#: VS001

Complete package

Our complete package provides antigen production (recombinant protein) and antibody production

• Recombinant protein production
• Immunization (5 mice/antigen) and Sera Testing by ELISA
• Fusion and Colony Screening
• Subcloning, Colony Expansion and Freezing
• Guaranteed western blot positive clones (2 clones)
• Antibody Purification from Ascites by Protein A/G (2 clones, 3mg each)

Price $ 6200    Cat#VS002

Relating websites:

• Visit our mirror website relating to antibody production, production of monoclonal antibodies, antibody, antibodies monoclonal, and hybridoma.
• Visit our company website www.vrisko.net.

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